Proteintech, 10675-1-AP, PAIP1 Polyclonal antibody

Size: 20ul / 150ul The PAIP1 (10675-1-AP) by Proteintech is a Polyclonal antibody targeting PAIP1 in WB, IF/ICC, IP, ELISA applications with reactivity to human, monkey samples 10675-1-AP targets PAIP1 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, monkey samples. Tested Applications Positive WB detected in: HeLa cells, BxPC-3 cells, COS-7 cells Positive IP detected in: HeLa cells Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information In initiation of translation in eukaryotes, binding of the small ribosomal subunit to mRNA requires recognition of the 5-prime cap structure by the cap-binding complex eIF4F. eIF4F consists of eIF4E, eIF4A, and eIF4G. Translation initiation is further regulated by the mRNA 3-prime poly(A) tail and the poly(A)-binding protein (PABC1). PAIP1 interacts with PABC1 and some eIF4 complexes [PMID:9548260]. It is a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs, and associates with EIF4A and PABPC1 may potentiate contacts between mRNA termini [PMID:11051545]. Specification Tested Reactivity: human, monkey Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1070 Product name: Recombinant human PAIP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 102-400 aa of BC005295 Sequence: EQFAETLNGCVTTDDALQELVELIYQQATSIPNFSYMGARLCNYLSHHLTISPQSGNFRQLLLQRCRTEYEVKDQAAKGDEVTRKRFHAFVLFLGELYLNLEIKGTNGQVTRADILQVGLRELLNALFSNPMDDNLICAVKLLKLTGSVLEDAWKEKGKMDMEEIIQRIENVVLDANCSRDVKQMLLKLVELRSSNWGRVHATSTYREATPENDPNYFMNEPTFYTSDGVPFTAADPDYQEKYQELLEREDFFPDYEENGTDLSGAGDPYLDDIDDEMDPEIEEAYEKFCLESERKRKQ Predict reactive species Full Name: poly(A) binding protein interacting protein 1 Calculated Molecular Weight: 70 kDa Observed Molecular Weight: 60 kDa, 51 kDa, 40 kDa GenBank Accession Number: BC005295 Gene Symbol: PAIP1 Gene ID (NCBI): 10605 RRID: AB_2160391 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9H074 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.