Proteintech, 10721-1-AP, CSRP3 Polyclonal antibody

Size: 20ul / 150ul The CSRP3 (10721-1-AP) by Proteintech is a Polyclonal antibody targeting CSRP3 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 10721-1-AP targets CSRP3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse heart tissue, human heart tissue, rat heart tissue Positive IP detected in: mouse heart tissue Positive IHC detected in: human heart tissue, human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information CSRP3/MLP (cysteine-rich protein 3/muscle Lim protein), a member of the cysteine-rich protein family, is a muscle-specific LIM-only factor specifically expressed in skeletal muscle. The cytoplasmic CSRP3 serves as a scaffold protein that primarily interacts and co-localizes with different Z-disc components of myofibrils (PMID: 31979369, 17519359). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1136 Product name: Recombinant human CSRP3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-194 aa of BC005900 Sequence: MPNWGGGAKCGACEKTVYHAEEIQCNGRSFHKTCFHCMACRKALDSTTVAAHESEIYCKVCYGRRYGPKGIGYGQGAGCLSTDTGEHLGLQFQQSPKPARSVTTSNPSKFTAKFGESEKCPRCGKSVYAAEKVMGGGKPWHKTCFRCAICGKSLESTNVTDKDGELYCKVCYAKNFGPTGIGFGGLTQQVEKKE Predict reactive species Full Name: cysteine and glycine-rich protein 3 (cardiac LIM protein) Calculated Molecular Weight: 21 kDa Observed Molecular Weight: 21 kDa GenBank Accession Number: BC005900 Gene Symbol: CSRP3 Gene ID (NCBI): 8048 RRID: AB_2292475 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P50461 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.