Proteintech, 10777-1-AP, Dopamine beta Hydroxylase Polyclonal antibody

Size: 20ul / 150ul The Dopamine beta Hydroxylase (10777-1-AP) by Proteintech is a Polyclonal antibody targeting Dopamine beta Hydroxylase in WB, IHC, IF/ICC, IF-Fro, FC (Intra), ELISA applications with reactivity to human, mouse samples 10777-1-AP targets Dopamine beta Hydroxylase in WB, IHC, IF/ICC, IF-Fro, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: SH-SY5Y cells, NIH/3T3 cells, mouse brain tissue Positive IHC detected in: mouse brain tissue, human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-Fro detected in: rat brain tissue Positive IF/ICC detected in: SH-SY5Y cells Positive FC (Intra) detected in: SH-SY5Y cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information DBH belongs to the copper type II ascorbate-dependent monooxygenase family and catalyzes the oxidative hydroxylation of DBH to norepinephrine. It is almost exclusively located in the adrenal medulla and the synaptic vesicles of postganglionic sympathetic neurons(PMID:11857564).The DBH is glycosylated (63--67 kDa) while still in the membrane(PMID:2325165) and it can form a dimer(PMID:8546710). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1196 Product name: Recombinant human DBH protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 269-603 aa of BC017174 Sequence: CDSKMKPDRLNYCRHVLAAWALGAKAFYYPEEAGLAFGGPGSSRYLRLEVHYHNPLVIEGRNDSSGIRLYYTAKLRRFNAGIMELGLVYTPVMAIPPRETAFILTGYCTDKCTQLALPPSGIHIFASQLHTHLTGRKVVTVLVRDGREWEIVNQDNHYSPHFQEIRMLKKVVSVHPGDVLITSCTYNTEDRELATVGGFGILEEMCVNYVHYYPQTQLELCKSAVDAGFLQKYFHLINRFNNEDVCTCPQASVSQQFTSVPWNSFNRDVLKALYSFAPISMHCNKSSAVRFQGEWNLQPLPKVISTLEEPTPQCPTSQGRSPAGPTVVSIGGGKG Predict reactive species Full Name: DBH Calculated Molecular Weight: 64.8 kDa Observed Molecular Weight: 63 kDa GenBank Accession Number: BC017174 Gene Symbol: DBH Gene ID (NCBI): 1621 RRID: AB_2277037 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P09172 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.