Proteintech, 10867-2-AP, OCT2/POU2F2 Polyclonal antibody

Size: 20ul / 150ul The OCT2/POU2F2 (10867-2-AP) by Proteintech is a Polyclonal antibody targeting OCT2/POU2F2 in WB, IHC, IP, ELISA applications with reactivity to human, mouse samples 10867-2-AP targets OCT2/POU2F2 in WB, IHC, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: NIH/3T3 cells, mouse ovary tissue Positive IP detected in: NIH/3T3 cells Positive IHC detected in: human lymphoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information POU2F2, also named as OCT2, is a 479 amino acid protein, which belongs to the POU transcription factor family. The Pit-Oct-Unc (POU) family of transcription factors includes many proteins that have been grouped in seven distinct classes according to function and expression containing proteins such as the neuronally expressed Pit proteins in class I, the ubiquitously expressed Oct-1 and tissue-specific Oct-2 in the second category, the well-known pluripotency marker Oct-4 which belongs to class V. Oct-1 (POU2f1) is a ubiquitous factor present in a wide range of cell types while Oct-2 (POU2f2) expression has been found confined to B lymphocytes and neuronal cells. Oct-2 has been implicated in B cell development and regulation of B lymphocyte-specific genes. POU2F2 exists various isoforms with MV 43, 50, and 62 kDa. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0955 Product name: Recombinant human POU2F2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 100-400 aa of BC006101 Sequence: AGDIQQLLQLQQLVLVPGHHLQPPAQFLLPQAQQSQPGLLPTPNLFQLPQQTQGALLTSQPRAGLPTQPPKCLEPPSHPEEPSDLEELEQFARTFKQRRIKLGFTQGDVGLAMGKLYGNDFSQTTISRFEALNLSFKNMCKLKPLLEKWLNDAETMSVDSSLPSPNQLSSPSLGFDGLPGRRRKKRTSIETNVRFALEKSFLANQKPTSEEILLIAEQLHMEKEVIRVWFCNRRQKEKRINPCSAAPMLPSPGKPASYSPHMVTPQGGAGTLPLSQASSSLSTTAQTPALKAATRLSACQA Predict reactive species Full Name: POU class 2 homeobox 2 Calculated Molecular Weight: 44 kDa Observed Molecular Weight: 55 kDa GenBank Accession Number: BC006101 Gene Symbol: POU2F2 Gene ID (NCBI): 5452 RRID: AB_2167080 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P09086 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.