Proteintech, 10917-2-AP, GATA1 Polyclonal antibody

Size: 20ul / 150ul The GATA1 (10917-2-AP) by Proteintech is a Polyclonal antibody targeting GATA1 in WB, IP, IHC, ELISA applications with reactivity to human, mouse samples 10917-2-AP targets GATA1 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HL-60 cells, Raji cells, K-562 cells, NIH/3T3 cells Positive IP detected in: K-562 cells Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information GATA1 is first identified as a member of the GATA transcription factor family, whose members bind the consensus (WGATAR) binding motif [PMID:22937757]. GATA1, a zinc finger DNA-binding transcription factor, plays a critical role in the normal development of hematopoietic cell lineages. The protein contains an N-terminal region that confers transcriptional activity and a C-terminal domain that mediates binding to DNA and other factors [PMID: 8524811]. GATA-1 is also implicated in regulating the expression of the erythroid and megakaryocytic-specific genes [PMID:22937757]. GATA1 exists two isoform with the molecular weight 50-55 kDa and 40-45 kDa (PMID: 31189107 ). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, zebrafish Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1350 Product name: Recombinant human GATA1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-316 aa of BC009797 Sequence: MEFPGLGSLGTSEPLPQFVDPALVSSTPESGVFFPSGPEGLDAAASSTAPSTATAAAAALAYYRDAEAYRHSPVFQVYPLLNCMEGIPGGSPYAGWAYGKTGLYPASTVCPTREDSPPQAVEDLDGKGSTSFLETLKTERLSPDLLTLGPALPSSLPVPNSAYGGPDFSSTFFSPTGSPLNSAAYSSPKLRGTLPLPPCEARECVNCGATATPLWRRDRTGHYLCNACGLYHKMNGQNRPLIRPKKRLIVSKRAGTQCTNCQTTTTTLWRRNASGDPVCNACGLYYKLHHQHYCGGSAQLMRAQSMASRGGVVSFS Predict reactive species Full Name: GATA binding protein 1 (globin transcription factor 1) Calculated Molecular Weight: 43 kDa Observed Molecular Weight: 50-55 kDa, 40-45 kDa GenBank Accession Number: BC009797 Gene Symbol: GATA1 Gene ID (NCBI): 2623 RRID: AB_2108279 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P15976 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.