Proteintech, 10964-2-AP, CDC34 Polyclonal antibody

Size: 20ul / 150ul The CDC34 (10964-2-AP) by Proteintech is a Polyclonal antibody targeting CDC34 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 10964-2-AP targets CDC34 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, human brain tissue, human pancreas tissue, HeLa cells Positive IP detected in: mouse testis tissue, HEK-293 cells Positive IHC detected in: human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information CDC34 is also named as UBCH3, UBE2R1(Ubiquitin-conjugating enzyme E2 R1) and belongs to the ubiquitin-conjugating enzyme family. Cdc34 enzyme is itself a substrate for the type of polyubiquitin modification that is known to cause targeting of proteins for degradation. It is phosphorylated in vivo, providing yet another potential means for control of its activity. And the predominantly nuclear localization of this enzyme suggests that substrate molecules relevant to its cell cycle function are similarly located within the nucleus(PMID:8164658). Human CDC34 could substitute efficiently for yeast CDC34(PMID:8248134). It can form a homodimer(PMID:8383676). This antibody can also recognize CDC34B(UBC3B) which is highly homologous to UBC3 (CDC34) with only 47 amino acid differences. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1438 Product name: Recombinant human CDC34 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-236 aa of BC009850 Sequence: MARPLVPSSQKALLLELKGLQEEPVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYFKARLKFPIDYPYSPPAFRFLTKMWHPNIYETGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMYRKWKESKGKDREYTDIIRKQVLGTKVDAERDGVKVPTTLAEYCVKTKAPAPDEGSDLFYDDYYEDGEVEEEADSCFGDDEDDSGTEES Predict reactive species Full Name: cell division cycle 34 homolog (S. cerevisiae) Calculated Molecular Weight: 34 kDa Observed Molecular Weight: 34 kDa GenBank Accession Number: BC009850 Gene Symbol: CDC34 Gene ID (NCBI): 997 RRID: AB_2077925 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P49427 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.