Proteintech, 11181-1-AP, NUDT6 Polyclonal antibody

Size: 20ul / 150ul The NUDT6 (11181-1-AP) by Proteintech is a Polyclonal antibody targeting NUDT6 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 11181-1-AP targets NUDT6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Jurkat cells, HepG2 cells, human heart tissue, rat heart tissue, rat liver tissue Positive IP detected in: HepG2 cells Positive IHC detected in: human liver tissue, mouse brain tissue, rat colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information NUDT6, also named as FGF2AS and GFG, belongs to the Nudix hydrolase family. It may contribute to the regulation of cell proliferation. NUDT6 maybe a candidate gene for fat deposition. The 72 kDa is a homodimer form of NUDT6. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1638 Product name: Recombinant human NUDT6 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-316 aa of BC009842 Sequence: MRQPLSWGRWRAMLARTYGPGPSAGYRWASGAQGYVRNPPVGACDLQGELDRFGGISVRLARLDALDRLDAAAFQKGLQAAVQQWRSEGRTAVWLHIPILQSRFIAPAASLGFCFHHAESDSSTLTLWLREGPSRLPGYASHQVGVAGAVFDESTRKILVVQDRNKLKNMWKFPGGLSEPEEDIGDTAVREVFEETGIKSEFRSVLSIRQQHTNPGAFGKSDMYIICRLKPYSFTINFCQEECLRCEWMDLNDLAKTENTTPITSRVARLLLYGYREGFDKIDLTVEELPAVYTGLFYKLYHKELPENYKTMKGID Predict reactive species Full Name: nudix (nucleoside diphosphate linked moiety X)-type motif 6 Calculated Molecular Weight: 36 kDa Observed Molecular Weight: 72 kDa GenBank Accession Number: BC009842 Gene Symbol: NUDT6 Gene ID (NCBI): 11162 RRID: AB_2236190 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P53370 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.