Proteintech, 11230-1-AP, Casein Kinase 1 Epsilon Polyclonal antibody

Size: 20ul / 150ul The Casein Kinase 1 Epsilon (11230-1-AP) by Proteintech is a Polyclonal antibody targeting Casein Kinase 1 Epsilon in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 11230-1-AP targets Casein Kinase 1 Epsilon in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A431 cells, mouse brain tissue Positive IHC detected in: human breast cancer tissue, human heart tissue, human kidney tissue, human testis tissue, human ovary tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information CSNK1E(Casein kinase I isoform epsilon) is also named as CKIe, CKI-epsilon and belongs to the casein kinase I subfamily. It plays a significant role in delaying the negative feedback signal within the transcription-translation-based autoregulatory loop that composes the core of the circadian mechanism and makes an ideal target for pharmaceutical compounds influencing circadian rhythms, sleep, and jet lag, as well as other physiologic and metabolic processes under circadian regulation(PMID:10775102). It can be autophosphorylated(PMID:19690332). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1742 Product name: Recombinant human CSNK1E protein Source: e coli. -derived, PKG Tag: GST Domain: 116-416 aa of BC006490 Sequence: IEYIHSKNFIHRDVKPDNFLMGLGKKGNLVYIIDFGLAKKYRDARTHQHIPYRENKNLTGTARYASINTHLGIEQSRRDDLESLGYVLMYFNLGSLPWQGLKAATKRQKYERISEKKMSTPIEVLCKGYPSEFSTYLNFCRSLRFDDKPDYSYLRQLFRNLFHRQGFSYDYVFDWNMLKFGAARNPEDVDRERREHEREERMGQLRGSATRALPPGPPTGATANRLRSAAEPVASTPASRIQPAGNTSPRAISRVDRERKVSMRLHRGAPANVSSSDLTGRQEVSRIPASQTSVPFDHLGK Predict reactive species Full Name: casein kinase 1, epsilon Calculated Molecular Weight: 47 kDa Observed Molecular Weight: 46-52 kDa GenBank Accession Number: BC006490 Gene Symbol: CK1 epsilon Gene ID (NCBI): 1454 RRID: AB_2084501 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P49674 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.