Proteintech, 11363-1-AP, ARHGEF1 Polyclonal antibody

Size: 20ul / 150ul The ARHGEF1 (11363-1-AP) by Proteintech is a Polyclonal antibody targeting ARHGEF1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 11363-1-AP targets ARHGEF1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, human brain tissue, human spleen tissue, Jurkat cells, K-562 cells, mouse spleen tissue Positive IP detected in: HEK-293 cells Positive IHC detected in: human colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A549 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1905 Product name: Recombinant human ARHGEF1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC011726 Sequence: MEDFARGAASPGPSRPGLVPVSIIGAEDEDFENELETNSEEQNSQFQSLEQVKRRPAHLMALLQHVALQFEPGPLVLRVPVPPNVAFELDRTRADLISEDVQRRFVQEVVQSQQVAVGRQLEDFRSKRLMGMTPWEQELAQLEAWVGRDRASYEARERHVAERLLMHLEEMQHTISTDEEKSAAVVNAIGLYMRHLGVRTKSGDKKSGRNFFRKKVMGNRRSDEPAKTKKGLSSILDAARWNRGEPQVPDFRHLKAEVDAEKPGATDRKGGVGMPSRDRNIGAPGQDTPGVSLHPLSLDSPDREPGADAPLELGDSSPQGPMSLESLAPPESTDEGAETESPEPGDEGEP Predict reactive species Full Name: Rho guanine nucleotide exchange factor (GEF) 1 Calculated Molecular Weight: 912 aa, 102 kDa Observed Molecular Weight: 102-110 kDa GenBank Accession Number: BC011726 Gene Symbol: ARHGEF1 Gene ID (NCBI): 9138 RRID: AB_2059741 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q92888 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.