Proteintech, 11397-1-AP, Septin 2 Polyclonal antibody

Size: 20ul / 150ul The Septin 2 (11397-1-AP) by Proteintech is a Polyclonal antibody targeting Septin 2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat samples 11397-1-AP targets Septin 2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, mouse heart tissue, HeLa cells, human skeletal muscle tissue, rat brain tissue, C6 cells, HEK-293 cells, K-562 cells, Jurkat cells Positive IP detected in: mouse brain tissue Positive IHC detected in: human liver cancer tissue, human colon tissue, human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells, hTERT-RPE1 cells and Mouse embryonic fibroblasts Positive FC (Intra) detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information SEPT2 belongs to a family of septins, GTP-binding proteins that are a component of the cytoskeleton. Septins can assemble into intracellular structures of various shapes and are known to influence membrane stability, forming diffusion barriers and serving as scaffolds.What is the molecular weight of SEPT2? Is SEPT2 post-translationally modified?The molecular weight of SEPT2 is 45 kDa. In a cellular context, SEPT2 forms hetero-hexamers and hetero-octamers with other septins. These hexamers and octamers further assemble into high-order structures such as filaments and rings. SEPT2 can be phosphorylated on Ser218 by casein kinase 2 (PMID: 19165576) and can also be SUMOylated (PMID: 29051266).What is the subcellular localization of SEPT2?SEPT2 forms a diffusion barrier and localizes to the primary cilium; in some cell types, it has been found at the transition zone/basal body (PMID: 20558667), while in others localized to the axoneme (PMID: 23572511). During cell division, SEPT2 accumulates near the contractile ring from anaphase through telophase, and finally condenses into the midbody (PMID: 9203580). In interphase cells, SEPT2 was found to associate with polyglutamylated microtubules (PMID: 18209106) and can affect microtubule dynamics via interaction with MAP4.What is the tissue expression pattern of SEPT2?While some septins have tissue-specific expression profiles, SEPT2 is ubiquitously expressed (PMID: 22314400). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, canine Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1946 Product name: Recombinant human SEPT2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-361 aa of BC014455 Sequence: MSKQQPTQFINPETPGYVGFANLPNQVHRKSVKKGFEFTLMVVGESGLGKSTLINSLFLTDLYPERVISGAAEKIERTVQIEASTVEIEERGVKLRLTVVDTPGYGDAINCRDCFKTIISYIDEQFERYLHDESGLNRRHIIDNRVHCCFYFISPFGHGLKPLDVAFMKAIHNKVNIVPVIAKADTLTLKERERLKKRILDEIEEHNIKIYHLPDAESDEDEDFKEQTRLLKASIPFSVVGSNQLIEAKGKKVRGRLYPWGVVEVENPEHNDFLKLRTMLITHMQDLQEVTQDLHYENFRSERLKRGGRKVENEDMNKDQILLEKEAELRRMQEMIARMQAQMQMQMQGGDGDGGALGHHV Predict reactive species Full Name: septin 2 Calculated Molecular Weight: 41 kDa Observed Molecular Weight: 45 kDa GenBank Accession Number: BC014455 Gene Symbol: Septin 2 Gene ID (NCBI): 4735 RRID: AB_2239110 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q15019 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.