Proteintech, 11639-1-AP, MRFAP1 Polyclonal antibody

Size: 20ul / 150ul The MRFAP1 (11639-1-AP) by Proteintech is a Polyclonal antibody targeting MRFAP1 in WB, IP, IHC, ELISA applications with reactivity to human samples 11639-1-AP targets MRFAP1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells Positive IP detected in: Jurkat cells Positive IHC detected in: human gliomas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information MRFAP1, also named as PAM14 and PGR1, is a 14 kDa protein which associated with MRG. It is found in a complex composed of MORF4L1, MRFAP1 and RB1. MRFAP1 interacts via its N-terminus with MORF4L1. And it interacts with CSTB and MORF4L2. Specification Tested Reactivity: human Cited Reactivity: human, monkey Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2227 Product name: Recombinant human MRFAP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-127 aa of BC022797 Sequence: MRPLDIVELAEPEEVEVLEPEEDFEQFLLPVINEMREDIASLTREHGRAYLRNRSKLWEMDNMLIQIKTQVEASEESALNHLQNPGDAAEGRAAKRCEKAEEKAKEIAKMAEMLVELVRRIEKSESS Predict reactive species Full Name: Mof4 family associated protein 1 Calculated Molecular Weight: 127 aa, 15 kDa Observed Molecular Weight: 15 kDa GenBank Accession Number: BC022797 Gene Symbol: MRFAP1 Gene ID (NCBI): 93621 RRID: AB_2145271 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y605 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.