Proteintech, 11655-2-AP, SSR4 Polyclonal antibody

Size: 20ul / 150ul The SSR4 (11655-2-AP) by Proteintech is a Polyclonal antibody targeting SSR4 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 11655-2-AP targets SSR4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, human placenta tissue, HepG2 cells, mouse liver tissue, rat liver tissue Positive IP detected in: HeLa cells Positive IHC detected in: human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information Signal sequence receptor subunit delta (SSR4, or TRAPD) gene encodes the translocon-associated proteins subunit delta, which is about 19-kDa and is located at endoplasmic reticulum (ER) membrane, the translocon-associated proteins complex functions to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. TRAP-delta can form heterotetramer with other TRAP homolog TRAP-alpha, TRAP-beta and TRAP-gamma. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2250 Product name: Recombinant human SSR4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-173 aa of BC003371 Sequence: MAAMASLGALALLLLSSLSRCSAEACLEPQITPSYYTTSDAVISTETVFIVEISLTCKNRVQNMALYADVGGKQFPVTRGQDVGRYQVSWSLDHKSAHAGTYEVRFFDEESYSLLRKAQRNNEDISIIPPLFTVSVDHRGTWNGPWVSTEVLAAAIGLVIYYLAFSAKSHIQA Predict reactive species Full Name: signal sequence receptor, delta (translocon-associated protein delta) Calculated Molecular Weight: 173 aa, 19 kDa Observed Molecular Weight: 19 kDa GenBank Accession Number: BC003371 Gene Symbol: SSR4 Gene ID (NCBI): 6748 RRID: AB_2195738 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P51571 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.