Proteintech, 11658-1-AP, CPI17 Polyclonal antibody

Size: 20ul / 150ul The CPI17 (11658-1-AP) by Proteintech is a Polyclonal antibody targeting CPI17 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 11658-1-AP targets CPI17 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: MCF-7 cells, HeLa cells, HepG2 cells, human colon tissue, Jurkat cells, PC-3 cells Positive IHC detected in: human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A549 cells Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information PPP1R14A, also named as CPI17, belongs to the protein phosphatase 1 (PP1) inhibitor family. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. CPI17 has two isoforms:CPI-17alpha (17 kDa) and CPI-17beta (13 kDa). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2237 Product name: Recombinant human PPP1R14A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-147 aa of BC021089 Sequence: MAAQRLGKRVLSKLQSPSRARGPGGSPGGLQKRHARVTVKYDRRELQRRLDVEKWIDGRLEELYRGMEADMPDEINIDELLELESEEERSRKIQGLLKSCGKPVEDFIQELLAKLQGLHRQPGLRQPSPSHDGSLSPLQDRARTAHP Predict reactive species Full Name: protein phosphatase 1, regulatory (inhibitor) subunit 14A Calculated Molecular Weight: 147 aa, 17 kDa Observed Molecular Weight: 17 kDa GenBank Accession Number: BC021089 Gene Symbol: CPI17 Gene ID (NCBI): 94274 RRID: AB_2252794 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96A00 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.