Proteintech, 11662-2-AP, VPS52 Polyclonal antibody

Size: 20ul / 150ul The VPS52 (11662-2-AP) by Proteintech is a Polyclonal antibody targeting VPS52 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 11662-2-AP targets VPS52 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HepG2 cells, COLO 320 cells, mouse skin tissue Positive IP detected in: mouse skin tissue Positive IHC detected in: human gliomas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information VPS52 is a component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54. GARP complex functions in traffic from endosomes the trans-Golgi network (PMID: 15878329). GARP proteins interact with RAB proteins and SNARE proteins. VPS52 may play a role in the development of a multicellular organism through cell-cell interactions (PMID: 23142660). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2180 Product name: Recombinant human VPS52 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 373-723 aa of BC032108 Sequence: EQRYPFEALFRSQHYALLDNSCREYLFICEFFVVSGPAAHDLFHAVMGRTLSMTLKHLDSYLADCYDAIAVFLCIHIVLRFRNIAAKRDVPALDRYWEQVLALLWPRFELILEMNVQSVRSTDPQRLGGLDTRPHYITRRYAEFSSALVSINQTIPNERTMQLLGQLQVEVENFVLRVAAEFSSRKEQLVFLINNYDMMLGVLMERAADDSKEVESFQQLLNARTQEFIEELLSPPFGGLVAFVKEAEALIERGQAERLRGEEARVTQLIRGFGSSWKSSVESLSQDVMRSFTNFRNGTSIIQGALTQLIQLYHRFHRVLSQPQLRALPARAELINIHHLMVELKKHKPNF Predict reactive species Full Name: vacuolar protein sorting 52 homolog (S. cerevisiae) Calculated Molecular Weight: 723 aa, 82 kDa Observed Molecular Weight: 82 kDa GenBank Accession Number: BC032108 Gene Symbol: VPS52 Gene ID (NCBI): 6293 RRID: AB_2241614 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8N1B4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.