Proteintech, 11726-2-AP, RAD1 Polyclonal antibody

Size: 20ul / 150ul The RAD1 (11726-2-AP) by Proteintech is a Polyclonal antibody targeting RAD1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 11726-2-AP targets RAD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, mouse spleen tissue, K-562 cells, L02 cells Positive IP detected in: HeLa cells Positive IHC detected in: human lung tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information RAD1, cell cycle checkpoint protein, is a component of a heterotrimeric cell cycle checkpoint 9-1-1 complex, that is activated to stop cell cycle progression in response to DNA damage or incomplete DNA replication. RAD1, HUS1 and RAD9 are central components of a DNA damage-responsive protein complex. Testis-specific disruption of RAD1 in mice resulted in defective double-strand break repair, depletion of germ cells and infertility (PMID: 35133274). RAD1 has 3 isoforms produced by alternative splicing. Specification Tested Reactivity: human, mouse Cited Reactivity: human, rat, rainbow trout Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2316 Product name: Recombinant human RAD1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-282 aa of BC006837 Sequence: MPLLTQQIQDEDDQYSLVASLDNVRNLSTILKAIHFREHATCFATKNGIKVTVENAKCVQANAFIQAGIFQEFKVQEESVTFRINLTVLLDCLSIFGSSPMPGTLTALRMCYQGYGYPLMLFLEEGGVVTVCKINTQEPEETLDFDFCSTNVINKIILQSEGLREAFSELDMTSEVLQITMSPDKPYFRLSTFGNAGSSHLDYPKDSDLMEAFHCNQTQVNRYKISLLKPSTKALVLSCKVSIRTDNRGFLSLQYMIRNEDGQICFVEYYCCPDEEVPESES Predict reactive species Full Name: RAD1 homolog (S. pombe) Calculated Molecular Weight: 32 kDa Observed Molecular Weight: 32 kDa GenBank Accession Number: BC006837 Gene Symbol: RAD1 Gene ID (NCBI): 5810 RRID: AB_2246457 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O60671 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.