Proteintech, 11730-1-AP, YPEL5 Polyclonal antibody

Size: 20ul / 150ul The YPEL5 (11730-1-AP) by Proteintech is a Polyclonal antibody targeting YPEL5 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 11730-1-AP targets YPEL5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, mouse testis tissue, rat brain tissue Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information YPEL5(Protein yippee-like 5) belongs to the yippee family and is involved in a certain cell division-related function.During cell cycle, YPEL5 protein is detected at different subcellular localizations; at interphase, it is located in the nucleus and centrosome, then it changes location sequentially to spindle poles, mitotic spindle, and spindle midzone during mitosis, and finally transferrs to midbody at cytokinesis(PMID:20580816). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, zebrafish Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2328 Product name: Recombinant human YPEL5 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-121 aa of BC000836 Sequence: MGRIFLDHIGGTRLFSCANCDTILTNRSELISTRFTGATGRAFLFNKVVNLQYSEVQDRVMLTGRHMVRDVSCKNCNSKLGWIYEFATEDSQRYKEGRVILERALVRESEGFEEHVPSDNS Predict reactive species Full Name: yippee-like 5 (Drosophila) Calculated Molecular Weight: 121 aa, 14 kDa Observed Molecular Weight: 14 kDa GenBank Accession Number: BC000836 Gene Symbol: YPEL5 Gene ID (NCBI): 51646 RRID: AB_2217465 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P62699 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.