Proteintech, 11750-1-AP, NFIA Polyclonal antibody

Size: 20ul / 150ul The NFIA (11750-1-AP) by Proteintech is a Polyclonal antibody targeting NFIA in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples 11750-1-AP targets NFIA in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A431 cells, HeLa cells, Jurkat cells, L02 cells, mouse liver tissue Positive IP detected in: A431 cells Positive IHC detected in: mouse brain tissue, human prostate cancer tissue, human gliomas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information The NFI (nuclear factor I) family consists of four members in vertebrates (NFI-A, NFI-B, NFI-C and NFI-X), and the four NFI genes are expressed in unique patterns during mouse embryogenesis and in the adult. Four isoforms of NFIA were found in human and they play various roles in DNA replication, DNA-dependent transcritpion via their DNA binding property. Multiple residues of NFIA can be phosphorylated resulting in mild shifts of its practical molecular weight. Recent finding also revealed its neuroprotective function in NMDA-induced neuronal damage. Catalog# 11750-1-AP is a rabbit polyclonal antibody raised against N-terminal of human original NFIA. The calculated molecular weight of NFIA is 55 kDa, However the size of the proteins cross-linked to the adenoviral NF-I element ranged from 60 to 80 kDa . The larger size observed by us could be due to the oligo protein complex, which would increase the size by 15-20 kDa. (PMID: 11447215) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2346 Product name: Recombinant human NFIA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-200 aa of BC022264 Sequence: MYSPLCLTQDEFHPFIEALLPHVRAFAYTWFNLQARKRKYFKKHEKRMSKEEERAVKDELLSEKPEVKQKWASRLLAKLRKDIRPEYREDFVLTVTGKKPPCCVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLVKSPQCSNPGLCVQPHHIGVSVKELDLYLAYFVHAADSSQSESPSQPSDA Predict reactive species Full Name: nuclear factor I/A Calculated Molecular Weight: 498 aa, 55 kDa Observed Molecular Weight: 60-70 kDa GenBank Accession Number: BC022264 Gene Symbol: NFIA Gene ID (NCBI): 4774 RRID: AB_2153214 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q12857 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.