Proteintech, 11926-1-AP, Kininogen 1 Polyclonal antibody

Size: 20ul / 150ul The Kininogen 1 (11926-1-AP) by Proteintech is a Polyclonal antibody targeting Kininogen 1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 11926-1-AP targets Kininogen 1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, A2780 cells, COLO 320 cells, MCF-7 cells, mouse kidney tissue Positive IHC detected in: human kidney tissue, human endometrial cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information Kininogens are inhibitors of thiol proteases. Kininogen 1 plays important role in Kinin-kallikrein system. This KNG1 gene contains 10 exons and encodes two proteins through alternative splicing: high molecular weight kininogen (HMWK) (72kDa) and low molecular weight kininogen (LMWK) (48kDa) (PMID: 37442062). HMWK is produced by the liver together with prekallikrein. It acts mainly as a cofactor on coagulation and inflammation, and has no intrinsic catalytic activity. LMWK is produced locally by numerous tissues, and secreted together with tissue kallikrein. 11926-1-AP was generated against N-terminal 300 aa of HMW kininogen. It can bind both HMW and LMW kiniogen. Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2574 Product name: Recombinant human KNG1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 20-374 aa of BC060039 Sequence: ESQSEEIDCNDKDLFKAVDAALKKYNSQNQSNNQFVLYRITEATKTVGSDTFYSFKYEIKEGDCPVQSGKTWQDCEYKDAAKAATGECTATVGKRSSTKFSVATQTCQITPAEGPVVTAQYDCLGCVHPISTQSPDLEPILRHGIQYFNNNTQHSSLFMLNEVKRAQRQVVAGLNFRMTYSIVQTNCSKENFLFLTPDCKSLWNGDTGECTDNAYIDIQLRIASFSQNCDIYPGKDFVQPPTKICVGCPRDIPTNSPELEETLTHTITKLNAENNATFYFKIDNVKKARVQVVAGKKYFIDFVARETTCSKESNEELTESCETKKLGQSLDCNAEVYVVPWEKKIYPTVNCQPLG Predict reactive species Full Name: kininogen 1 Calculated Molecular Weight: 644 aa, 72 kDa Observed Molecular Weight: 48 kDa, 72 kDa GenBank Accession Number: BC060039 Gene Symbol: Kininogen 1 Gene ID (NCBI): 3827 RRID: AB_2133403 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P01042 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.