Proteintech, 11970-1-AP, HSFY1 Polyclonal antibody

Size: 20ul / 150ul The HSFY1 (11970-1-AP) by Proteintech is a Polyclonal antibody targeting HSFY1 in WB, IHC, ELISA applications with reactivity to human samples 11970-1-AP targets HSFY1 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human testis tissue Positive IHC detected in: human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information The HSFY gene is located in the male-specific region of chromosome Y (MSY), which is not involved in X-Y crossover events. HSFY gene is expressed exclusively in testis. HSFY is a DNA-binding protein that specifically binds heat shock promoter elements(HSE), and suggested to be implicated in spermatogenesis both in animals and humans. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2592 Product name: Recombinant human HSFY1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-214 aa of BC055414 Sequence: MAHVSSETQDVSPKDELTASEASTRSPLCEHTFPGDSDLRSMIEEHAFQVLSQGSLLESPSYTVCVSEPDKDDDFLSLNFPRKLWKIVESDQFKSISWDENGTCIVINEELFKKEILETKAPYRIFQTDAIKSFVRQLNLYGFSKIQQNFQRSAFLATFLSEEKESSVLSKLHVFVFHHSLFGATFGEHHFQPCPLPSGNKAPISTWSGDSWLL Predict reactive species Full Name: heat shock transcription factor, Y-linked 1 Calculated Molecular Weight: 401 aa, 45 kDa Observed Molecular Weight: 45 kDa GenBank Accession Number: BC055414 Gene Symbol: HSFY1 Gene ID (NCBI): 86614 RRID: AB_2877810 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96LI6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.