Proteintech, 11998-1-AP, URG4 Polyclonal antibody

Size: 20ul / 150ul The URG4 (11998-1-AP) by Proteintech is a Polyclonal antibody targeting URG4 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 11998-1-AP targets URG4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells Positive IP detected in: HEK-293 cells Positive IHC detected in: human stomach cancer tissue, human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:200-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information URG4 (upregulated gene 4), also known as upregulator of cell proliferation (URGCP), is an oncogene which is involved in the development and progression of various tumors. URG4 is upregulated in hepatocellular carcinoma (HCC), ovarian cancer, and other types of cancer. Overexpression of URG4 not only results in tumour progression but also in metastasis and recurrence. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2630 Product name: Recombinant human URG4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-358 aa of BC018426 Sequence: MASPGHSDLGEVAPEIKASERRTAVAIADLEWREMEGDDCEFRYGDGTNEAQDNDFPTVERSRLQEMLSLLGLETYQVQKLSLQDSLQISFDSMKNWAPQVPKDLPWNFLRKLQALNADARNTTMVLDVLPDARPVEKESQMEEEIIYWDPADDLAADIYSFSELPTPDTPVNPLDLLCALLLSSDSFLQQEIALKMALCQFALPLVLPDSENHYHTFLLWAMRGIVRTWWSQPPRGMGSFREDSVVLSRAPAFAFVRMDVSSNSKSQLLNAVLSPGHRQWDCFWHRDLNLGTNAREISDGLVEISWFFPSGREDLDIFPEPVAFLNLRGDIGSHWLQFKLLTEISSAVFILTDNISK Predict reactive species Full Name: up-regulated gene 4 Calculated Molecular Weight: 922 aa, 104 kDa Observed Molecular Weight: 104 kDa GenBank Accession Number: BC018426 Gene Symbol: URG4 Gene ID (NCBI): 55665 RRID: AB_1072845 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8TCY9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.