Proteintech, 12004-1-AP, EFEMP2 Polyclonal antibody

Size: 20ul / 150ul The EFEMP2 (12004-1-AP) by Proteintech is a Polyclonal antibody targeting EFEMP2 in WB, IHC, IP, ELISA applications with reactivity to human, mouse samples 12004-1-AP targets EFEMP2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HT-29 cells, K-562 cells, mouse kidney tissue, JAR cells, SW480 cells, human heart tissue Positive IP detected in: mouse kidney tissue Positive IHC detected in: human heart tissue, human prostate cancer tissue, human skin tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information EFEMP2, also known as Fibulin-4 is a 60 kDa extracellular matrix (ECM) glycoprotein that is a pivotal oncogene in tumor progression and metastasis. EFEMP2 is necessary for elastic fiber formation and connective tissue development. Defects in this gene are cause of an autosomal recessive cutis laxa syndrome. Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2640 Product name: Recombinant human EFEMP2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 144-443 aa of BC010456 Sequence: PGSYQCTCPDGYRKIGPECVDIDECRYRYCQHRCVNLPGSFRCQCEPGFQLGPNNRSCVDVNECDMGAPCEQRCFNSYGTFLCRCHQGYELHRDGFSCSDIDECSYSSYLCQYRCVNEPGRFSCHCPQGYQLLATRLCQDIDECESGAHQCSEAQTCVNFHGGYRCVDTNRCVEPYIQVSENRCLCPASNPLCREQPSSIVHRYMTITSERSVPADVFQIQATSVYPGAYNAFQIRAGNSQGDFYIRQINNVSAMLVLARPVTGPREYVLDLEMVTMNSLMSYRASSVLRLTVFVGAYTF Predict reactive species Full Name: EGF-containing fibulin-like extracellular matrix protein 2 Calculated Molecular Weight: 443 aa, 49 kDa Observed Molecular Weight: 60 kDa GenBank Accession Number: BC010456 Gene Symbol: EFEMP2 Gene ID (NCBI): 30008 RRID: AB_10667002 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O95967 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.