Proteintech, 12006-1-AP, VPS45 Polyclonal antibody

Size: 20ul / 150ul The VPS45 (12006-1-AP) by Proteintech is a Polyclonal antibody targeting VPS45 in IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 12006-1-AP targets VPS45 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive IHC detected in: mouse brain tissue, human testis tissue, human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells, HepG2 cells Recommended dilution Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information The Sec1/Munc18 (SM) family of proteins regulate intracellular trafficking through interactions with individual SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor) proteins and assembled SNARE complexes. The SM protein VPS45 (Vacuolar protein sorting-associated protein 45) has a calculated molecular mass of 65 kDa and shares 38% identity with yeast Vps45 which is required for the formation of yeast endosomal SNARE complexes and is thus essential for traffic through the endosomal system. VPS45 may play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network. (PMID: 8996080; 23166732) Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2658 Product name: Recombinant human VPS45 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 221-570 aa of BC012932 Sequence: DAITPLLNQWTYQAMVHELLGINNNRIDLSRVPGISKDLREVVLSAENDEFYANNMYLNFAEIGSNIKNLMEDFQKKKPKEQQKLESIADMKAFVENYPQFKKMSGTVSKHVTVVGELSRLVSERNLLEVSEVEQELACQNDHSSALQNIKRLLQNPKVTEFDAARLVMLYALHYERHSSNSLPGLMMDLRNKGVSEKYRKLVSAVVEYGGKRVRGSDLFSPKDAVAITKQFLKGLKGVENVYTQHQPFLHETLDHLIKGRLKENLYPYLGPSTLRDRPQDIIVFVIGGATYEEALTVYNLNRTTPGVRIVLGGTTVHNTKSFLEEVLASGLHSRSKESSQVTSRSASRR Predict reactive species Full Name: vacuolar protein sorting 45 homolog (S. cerevisiae) Calculated Molecular Weight: 570 aa, 65 kDa GenBank Accession Number: BC012932 Gene Symbol: VPS45 Gene ID (NCBI): 11311 RRID: AB_2215369 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NRW7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.