Proteintech, 12043-1-AP, NOL8 Polyclonal antibody

Size: 20ul / 150ul The NOL8 (12043-1-AP) by Proteintech is a Polyclonal antibody targeting NOL8 in WB, IP, ELISA applications with reactivity to human, mouse, rat samples 12043-1-AP targets NOL8 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: COLO 320 cells, Jurkat cells Positive IP detected in: COLO 320 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information NOL8 (Nucleolar Protein 8) plays an essential role in the survival of diffuse-type gastric cancer cells,and knocking down NOL8 promoted cell apoptosis. The structure analysis suggested that NOL8 contains a RNA binding motif, which was consistent with the study that NOL8 interacted with DEAD-box RNA helicase Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2672 Product name: Recombinant human NOL8 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 817-1147 aa of BC013788 Sequence: GEEWVKESMGKTSGKLFDSSDDDESDSEDDSNRFKIKPQFEGRAGQKLMDLQSHFGTDDRFRMDSRFLETDSEEEQEEVNEKKTAEEEELAEEKKKALNVVQSVLQINLSNSTNRGSVAAKKFKDIIHYDPTKQDHATYERKRDDKPKESKAKRKKKREEAEKLPEVSKEMYYNIAMDLKEIFQTTKYTSEKEEGTPWNEDCGKEKPEEIQDPAALTSDAEQPSGFTFSFFDSDTKDIKEETYRVETVKPGKIVWQEDPRLQDSSSEEEDVTEETDHRNSSPGEASLLEKETTRFFFFSKNDERLQGSDLFWRGVGSNMSRNSWEARTTNLRM Predict reactive species Full Name: nucleolar protein 8 Calculated Molecular Weight: 132 kDa Observed Molecular Weight: 150 kDa GenBank Accession Number: BC013788 Gene Symbol: NOL8 Gene ID (NCBI): 55035 RRID: AB_2152001 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q76FK4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.