Proteintech, 12050-1-AP, SLU7 Polyclonal antibody

Size: 20ul / 150ul The SLU7 (12050-1-AP) by Proteintech is a Polyclonal antibody targeting SLU7 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 12050-1-AP targets SLU7 in WB, IF, RIP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Jurkat cells, HeLa cells, mouse colon tissue, K-562 cells, Y79 cells Positive IHC detected in: mouse pancreas tissue, human colon cancer tissue, rat pancreas tissue, rat stomach tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Pre-mRNA splicing occurs in two sequential transesterification steps. SLU7 is a splicing factor that has been found to be essential during the second catalytic step in the pre-mRNA splicing process. It associates with the spliceosome and contains a zinc knuckle motif that is found in other splicing factors and is involved in protein-nucleic acid and protein-protein interactions. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2680 Product name: Recombinant human SLU7 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-301 aa of BC010634 Sequence: MSATVVDAVNAAPLSGSKEMSLEEPKKMTREDWRKKKELEEQRKLGNAPAEVDEEGKDINPHIPQYISSVPWYIDPSKRPTLKHQRPQPEKQKQFSSSGEWYKRGVKENSVITKYRKGACENCGAMTHKKKDCFERPRRVGAKFTGTNIAPDEHVQPQLMFDYDGKRDRWNGYNPEEHMKIVEEYAKVDLAKRTLKAQKLQEELASGKLVEQANSPKHQWGEEEPNSQTEKDHNSEDEDEDKYADDIDMPGQNFDSKRRITVRNLRIREDIAKYLRNLDPNSAYYDPKTRAMRENPYANAG Predict reactive species Full Name: SLU7 splicing factor homolog (S. cerevisiae) Calculated Molecular Weight: 586 aa, 68 kDa Observed Molecular Weight: 68 kDa GenBank Accession Number: BC010634 Gene Symbol: SLU7 Gene ID (NCBI): 10569 RRID: AB_10663102 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O95391 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.