Proteintech, 12054-1-AP, PSMD3 Polyclonal antibody

Size: 20ul / 150ul The PSMD3 (12054-1-AP) by Proteintech is a Polyclonal antibody targeting PSMD3 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 12054-1-AP targets PSMD3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A549 cells, human brain tissue, human kidney tissue, mouse brain tissue, HEK-293 cells, mouse kidney tissue, rat kidney tissue Positive IP detected in: mouse kidney tissue Positive IHC detected in: human colon cancer tissue, human breast cancer tissue, human small intestine tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:250-1:1000 Background Information PSMD3, encoding proteasome (prosome, macropain) 26S subunit, non-ATPase 3, a member of the 26S proteasome family. PSMD3 is expressed in most of the tissues according to the RNA-Seq database. It has been reported that PSMD3 genotypes are associated with white blood cell (WBC) and neutrophil counts and have interactions with dietary fatty acids and carbohydrates on glucose-related traits. PSMD3 is located at ch17q21 and encodes a protein with a molecular weight of 61 kDa (PMID: 31013812). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2684 Product name: Recombinant human PSMD3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 235-534 aa of BC012302 Sequence: LRTATLRHDADGQATLLNLLLRNYLHYSLYDQAEKLVSKSVFPEQANNNEWARYLYYTGRIKAIQLEYSVARRTMTNALRKAPQHTAVGFKQTVHKLLIVVELLLGEIPDRLQFRQPSLKRSLMPYFLLTQAVRTGNLAKFNQVLDQFGEKFQADGTYTLIIRLRHNVIKTGVRMISLSYSRISLADIAQKLQLDSPEDAEFIVAKAIRDGVIEASINHEKGYVQSKEMIDIYSTREPQLAFHQRISFCLDIHNMSVKAMRFPPKSYNKDLESAEERREREQQDLEFAKEMAEDDDDSFP Predict reactive species Full Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 3 Calculated Molecular Weight: 534 aa, 61 kDa Observed Molecular Weight: 61 kDa GenBank Accession Number: BC012302 Gene Symbol: PSMD3 Gene ID (NCBI): 5709 RRID: AB_2170484 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O43242 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.