Proteintech, 12147-1-AP, GAK Polyclonal antibody

Size: 20ul / 150ul The GAK (12147-1-AP) by Proteintech is a Polyclonal antibody targeting GAK in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 12147-1-AP targets GAK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: human brain tissue Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information GAK(cyclin-G-associated kinase) belongs to the protein kinase superfamily. GAK plays a more positive role as a modulator of signal transduction, by relaying the phosphorylation signal from an unidentified tyrosine kinase to unidentified GAK phosphorylation targets(PMID:9013862). It may be acting as an auxilin homolog that is involved in the uncoating of clathrin- coated vesicles by hsc70 in non-neuronal cells. Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2795 Product name: Recombinant human GAK protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-309 aa of BC008668 Sequence: MSLLQSALDFLAGPGSLGGASGRDQSDFVGQTVELGELRLLLASPAPPLSVQSTPRGGPPAAADPFGPLLPSSGNNSQPCSNPDLFGEFLNSDSVTVPPSFPSAHSSPPPSCSADFLHLGDLPGEPSKMTASSSNPDLLGGWAAWTETAASAVAPTPATEGPLFSPGGQPAPCGSQASWTKSQNPDPFADLGDLSSGLQGSPAGFPPGGFIPKTATTPKGSSSWQTSRPPAQGASWPPQAKPPPKACTQPRPNYASNFSVIGAREERGVRAPSFAQKPKVSENDFEDLLSNQGFSSRSDKKGPKTIAEM Predict reactive species Full Name: cyclin G associated kinase Calculated Molecular Weight: 134 kDa, 143 kDa Observed Molecular Weight: 150 kDa GenBank Accession Number: BC008668 Gene Symbol: GAK Gene ID (NCBI): 2580 RRID: AB_2108368 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O14976 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.