Proteintech, 12230-2-AP, RBBP9 Polyclonal antibody

Size: 20ul / 150ul The RBBP9 (12230-2-AP) by Proteintech is a Polyclonal antibody targeting RBBP9 in WB, IHC, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat samples 12230-2-AP targets RBBP9 in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse testis tissue, A375 cells, human lung tissue, human spleen tissue, mouse lung tissue Positive IP detected in: mouse lung tissue Positive IHC detected in: human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: BxPC-3 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information RBBP9, also named as BOG, RBBP10, RBBP-9, RBBP-10 and Protein BOG, belongs to the RBBP9 family. It may play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-β1 through interaction with retinoblastoma and the subsequent displacement of E2F-1. RBBP9 is a tumor-associated serine hydrolase activity required for pancreatic neoplasia. It mediates suppression of TGF-β signaling is required for E-cadherin expression as loss of the serine hydrolase activity leads to a reduction in E-cadherin levels and a concomitant decrease in the integrity of tumor cell-cell junctions..RBBP9 protein levels were equivalent in paired primary tumor and nonneoplastic specimens(PMID: 20080647) The genes CyFIP2 and RbBP9, which are also miss-expressed in ALS hMSC, could serve as diagnostic biomarker tools for detection of ALS in blood samples. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2868 Product name: Recombinant human RBBP9 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-186 aa of BC015938 Sequence: MASPSKAVIVPGNGGGDVTTHGWYGWVKKELEKIPGFQCLAKNMPDPITARESIWLPFMETELHCDEKTIIIGHSSGAIAAMRYAETHRVYAIVLVSAYTSDLGDENERASGYFTRPWQWEKIKANCPYIVQFGSTDDPFLPWKEQQEVADRLETKLHKFTDCGHFQNTEFHELITVVKSLLKVPA Predict reactive species Full Name: retinoblastoma binding protein 9 Calculated Molecular Weight: 186 aa, 21 kDa Observed Molecular Weight: 22 kDa GenBank Accession Number: BC015938 Gene Symbol: RBBP9 Gene ID (NCBI): 10741 RRID: AB_2175265 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O75884 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.