Proteintech, 12263-1-AP, CCT8 Polyclonal antibody

Size: 20ul / 150ul The CCT8 (12263-1-AP) by Proteintech is a Polyclonal antibody targeting CCT8 in WB, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 12263-1-AP targets CCT8 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A431 cells, mouse kidney tissue, HEK-293 cells, COLO 320 cells, HeLa cells, Jurkat cells, Raji cells Positive IP detected in: Jurkat cells Positive IF/ICC detected in: SW480 cells, MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information CCT8, also named as C21orf112, CCTQ, KIAA0002, CCT-theta, TCP-1-theta and NY-REN-15, belongs to the TCP-1 chaperonin family. It is a molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, CCT8 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.(PMID:20080638) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2926 Product name: Recombinant human CCT8 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 146-497 aa of BC012584 Sequence: FNVDNIRVCKILGSGISSSSVLHGMVFKKETEGDVTSVKDAKIAVYSCPFDGMITETKGTVLIKTAEELMNFSKGEENLMDAQVKAIADTGANVVVTGGKVADMALHYANKYNIMLVRLNSKWDLRRLCKTVGATALPRLTPPVLEEMGHCDSVYLSEVGDTQVVVFKHEKEDGAISTIVLRGSTDNLMDDIERAVDDGVNTFKVLTRDKRLVPGGGATEIELAKQITSYGETCPGLEQYAIKKFAEAFEAIPRALAENSGVKANEVISKLYAVHQEGNKNVGLDIEAEVPAVKDMLEAGILDTYLGKYWAIKLATNAAVTVLRVDQIIMAKPAGGPKPPSGKKDWDDDQND Predict reactive species Full Name: chaperonin containing TCP1, subunit 8 (theta) Calculated Molecular Weight: 548 aa, 60 kDa Observed Molecular Weight: 60 kDa GenBank Accession Number: BC012584 Gene Symbol: CCT8 Gene ID (NCBI): 10694 RRID: AB_906331 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P50990 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.