Proteintech, 12280-1-AP, ETS2 Polyclonal antibody

Size: 20ul / 150ul The ETS2 (12280-1-AP) by Proteintech is a Polyclonal antibody targeting ETS2 in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples 12280-1-AP targets ETS2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: K-562 cells, HeLa cells, MCF-7 cells, mouse kidney tissue, PC-3 cells Positive IP detected in: HeLa cells Positive IHC detected in: mouse spleen tissue, rat liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2929 Product name: Recombinant human ETS2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 84-444 aa of BC017040 Sequence: KATFSGFKKEQRRLGIPKNPWLWSEQQVCQWLLWATNEFSLVNVNLQRFGMNGQMLCNLGKERFLELAPDFVGDILWEHLEQMIKENQEKTEDQYEENSHLTSVPHWINSNTLGFGTEQAPYGMQTQNYPKGGLLDSMCPASTPSVLSSEQEFQMFPKSRLSSVSVTYCSVSQDFPGSNLNLLTNNSGTPKDHDSPENGADSFESSDSLLQSWNSQSSLLDVQRVPSFESFEDDCSQSLCLNKPTMSFKDYIQERSDPVEQGKPVIPAAVLAGFTGSGPIQLWQFLLELLSDKSCQSFISWTGDGWEFKLADPDEVARRWGKRKNKPKMNYEKLSRGLRYYYDKNIIHKTSGKRYVYRFVC Predict reactive species Full Name: v-ets erythroblastosis virus E26 oncogene homolog 2 (avian) Calculated Molecular Weight: 469 aa, 53 kDa Observed Molecular Weight: 53 kDa GenBank Accession Number: BC017040 Gene Symbol: ETS2 Gene ID (NCBI): 2114 RRID: AB_10804755 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P15036 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.