Proteintech, 12305-2-AP, APLP1 Polyclonal antibody

Size: 20ul / 150ul The APLP1 (12305-2-AP) by Proteintech is a Polyclonal antibody targeting APLP1 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 12305-2-AP targets APLP1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, rat brain tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information APLP1 (Amyloid Precursor-Like Protein 1) is a member of the amyloid precursor protein (APP) family, which also includes APP and APLP2. It is a type I transmembrane glycoprotein that is highly and predominantly expressed in the central nervous system, especially at the postsynaptic density of neurons. Functionally, APLP1 plays important roles in synaptic adhesion, synaptogenesis, and neurite outgrowth. It can form homophilic trans-interactions between neighboring cells, contributing to cell-cell adhesion and the formation of synaptic structures. Its proteolytic processing by α-, β-, and γ-secretases releases intracellular fragments that may participate in transcriptional regulation and signal transduction, potentially influencing gene expression and neuronal survival. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2951 Product name: Recombinant human APLP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 55-341 aa of BC012889 Sequence: QVAGLCGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYPELQIARVEQATQAIPMERWCGGSRSGSCAHPHHQVVPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPGSRVEGAEDEEEEESFPQPVDDYFVEPPQAEEEEETVPPPSSHTLAVVGKVTPTPRPTDGVDIYFGMPGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKN Predict reactive species Full Name: amyloid beta (A4) precursor-like protein 1 Calculated Molecular Weight: 650 aa, 72 kDa Observed Molecular Weight: 72 kDa GenBank Accession Number: BC012889 Gene Symbol: APLP1 Gene ID (NCBI): 333 RRID: AB_2056369 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P51693 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.