Proteintech, 12330-2-AP, TAK1 Polyclonal antibody

Size: 20ul / 150ul The TAK1 (12330-2-AP) by Proteintech is a Polyclonal antibody targeting TAK1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, hamster samples 12330-2-AP targets TAK1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, hamster samples. Tested Applications Positive WB detected in: NIH/3T3 cells, K-562 cells, SKOV-3 cells, COLO 320 cells, A375 cells, A431 cells, CHO cells, MCF-7 cells, HeLa cells, mouse heart tissue, A2780 cells Positive IP detected in: NIH/3T3 cells Positive IHC detected in: human prostate cancer tissue, human brain tissue, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:100-1:400 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Transforming growth factor-β (TGF-β)-activated kinase 1 (TAK1), also known as MAP3K7-mitogen-activatedprotein kinase kinase kinase 7-  is a serine/threonine  protein kinase triggering phosphorylation ofAMP-activatedprotein kinase (AMPK) and playing a role in the signal transduction of TGF-β, bone morphogenetic  proteins(BMPs), and various proinflammatory mediators.What is the molecular weight of TAK1? Are there any isoforms of TAK1?The molecular weight of full-length TAK1 is 55 kDa. There are four splicing variants of TAK1. TAK1-Brepresentsthe full-length variant, while TAK1-A, TAK1-C, and TAK1-D lack exons 12and/or 16.TAK-1variants differ in theirexpression levels across tissues (PMID: 11118615).Is TAK1 post-translationally modified?TAK1 kinase activity is modulated by interacting with TAK1-bindingproteins (TABs). Binding  to  TABs  triggersautophosphorylation of TAK1 and depends on  polyubiquitination of receptor-interacting protein 1 (RIP1)(PMID: 8638164 and 11460167). Additionally, TAK1 can be ubiquitinated, acetylated, and O-glycosylated,which impacts its activity (PMID: 28106845).What is the subcellular localization of TAK1?TAK1 is present in the cytoplasm.What is the tissue expression pattern of TAK1?TAK1 is ubiquitously expressed.How can TAK1 activity be monitored?TAK1 requires phosphorylation for its activity. TAK1 autophosphorylation in the presence of TBPs occurs onThr178,Thr184, Thr187, and Ser192 residues. The most commonly used phosphorylation readout is Thr187phosphorylation. Specification Tested Reactivity: human, mouse, hamster Cited Reactivity: human, mouse, rat, pig, fish Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3041 Product name: Recombinant human MAP3K7 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-291 aa of BC017715 Sequence: MSTASAASSSSSSSAGEMIEAPSQVLNFEEIDYKEIEVEEVVGRGAFGVVCKAKWRAKDVAIKQIESESERKAFIVELRQLSRVNHPNIVKLYGACLNPVCLVMEYAEGGSLYNVLHGAEPLPYYTAAHAMSWCLQCSQGVAYLHSMQPKALIHRDLKPPNLLLVAGGTVLKICDFGTACDIQTHMTNNKGSAAWMAPEVFEGSNYSEKCDVFSWGIILWEVITRRKPFDEIGGPAFRIMWAVHNGTRPPLIKNLPKPIESLMTRCWSKDPSQRPSMEEIVKIMTHLMRYF Predict reactive species Full Name: mitogen-activated protein kinase kinase kinase 7 Calculated Molecular Weight: 579 aa, 64 kDa Observed Molecular Weight: 64-67 kDa, 75-80 kDa GenBank Accession Number: BC017715 Gene Symbol: TAK1 Gene ID (NCBI): 6885 RRID: AB_2140101 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O43318 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.