Proteintech, 12352-1-AP, GILZ Polyclonal antibody

Size: 20ul / 150ul The GILZ (12352-1-AP) by Proteintech is a Polyclonal antibody targeting GILZ in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 12352-1-AP targets GILZ in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue Positive IHC detected in: mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information GILZ, also known as TSC22D3 and DSIPI, is a glucocorticoid responsive protein and is thought to mediate some of the anti-inflammatory effects of glucocorticoid receptors (GRs). GILZ is mainly localized in the cytoplasm. It is an anti-inflammatory molecule that could mediate the immunomodulatory activities of GCs and also plays a role in cell differentiation in various cells and tissues (PMID: 35011684). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3015 Product name: Recombinant human TSC22D3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-92 aa of BC018148 Sequence: SGASVVAIDNKIEQAMDLVKNHLMYAVREEVEILKEQIRELVEKNSQLERENTLLKTLASPEQLEKFQSCLSPEEPAPESPQVPEAPGGSAV Predict reactive species Full Name: TSC22 domain family, member 3 Calculated Molecular Weight: 134 aa, 15 kDa Observed Molecular Weight: 17 kDa GenBank Accession Number: BC018148 Gene Symbol: GILZ Gene ID (NCBI): 1831 RRID: AB_2272196 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q99576 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.