Proteintech, 12362-1-AP, DNAJC1 Polyclonal antibody

Size: 20ul / 150ul The DNAJC1 (12362-1-AP) by Proteintech is a Polyclonal antibody targeting DNAJC1 in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples 12362-1-AP targets DNAJC1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: MCF-7 cells, A549 cells, Raji cells, NIH/3T3 cells Positive IP detected in: Raji cells Positive IHC detected in: human breast cancer tissue, human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3030 Product name: Recombinant human DNAJC1 protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 194-554 aa of BC030955 Sequence: TGSKSVDVSKLGASEKNERLLMKPQWHDLLPCKLGIWFCLTLKALPHLIQDAGQFYAKYKETRLKEKEDALTRTELETLQKQKKVKKPKPEFPVYTPLETTYIQSYDHGTSIEEIEEQMDDWLENRNRTQKKQAPEWTEEDLSQLTRSMVKFPGGTPGRWEKIAHELGRSVTDVTTKAKQLKDSVTCSPGMVRLSELKSTVQNSRPIKTATTLPDDMITQREDAEGVAAEEEQEGDSGEQETGATDARPRRRKPARLLEATAKPEPEEKSRAKRQKDFDIAEQNESSDEESLRKERARSAEEPWTQNQQKLLELALQQYPRGSSDCWDKIARCVPSKSKEDCIARYKLLVELVQKKKQAKS Predict reactive species Full Name: DnaJ (Hsp40) homolog, subfamily C, member 1 Calculated Molecular Weight: 554 aa, 64 kDa Observed Molecular Weight: 64 kDa GenBank Accession Number: BC030955 Gene Symbol: DNAJC1 Gene ID (NCBI): 64215 RRID: AB_2094754 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96KC8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.