Proteintech, 12370-1-AP, TSKU Polyclonal antibody

Size: 20ul / 150ul The TSKU (12370-1-AP) by Proteintech is a Polyclonal antibody targeting TSKU in WB, IHC, ELISA applications with reactivity to human, mouse samples 12370-1-AP targets TSKU in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse liver tissue, HepG2 cells, mouse colon tissue, mouse pancreas tissue Positive IHC detected in: human colon cancer tissue, human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information TSK, also known as Tsukushi, is an atypical member of the small leucine-rich proteoglycan (SLRP) family of extracellular matrix proteins (PMID: 16884925). The human TSK cDNA encodes a 16 amino acid (aa) signal sequence and a 337 aa secreted protein that contains nine LRR, which has a calculated molecular mass of 38kDa and an apparent molecular mass of 40-50kDa cause of the N-glycosylation. In chick embryos, C-TSK is a unique BMP inhibitor that forms a ternary complex with BMP and chordin and is involved in the regulation of primitive streak and node formation. C-TSK migrates as a band of 47 kDa in SDS-PAGE and N-glycosidase F treatment reduces the size to 41 kDa (PMID: 15363410). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3054 Product name: Recombinant human TSKU protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-353 aa of BC020975 Sequence: MPWPLLLLLAVSGAQTTRPCFPGCQCEVETFGLFDSFSLTRVDCSGLGPHIMPVPIPLDTAHLDLSSNRLEMVNESVLAGPGYTTLAGLDLSHNLLTSISPTAFSRLRYLESLDLSHNGLTALPAESFTSSPLSDVNLSHNQLREVSVSAFTTHSQGRALHVDLSHNLIHRLVPHPTRAGLPAPTIQSLNLAWNRLHAVPNLRDLPLRYLSLDGNPLAVIGPGAFAGLGGLTHLSLASLQRLPELAPSGFRELPGLQVLDLSGNPKLNWAGAEVFSGLSSLQELDLSGTNLVPLPEALLLHLPALQSVSVGQDVRCRRLVREGTYPRRPGSSPKVALHCVDTRDSAARGPTIL Predict reactive species Full Name: tsukushin Calculated Molecular Weight: 353 aa, 38 kDa Observed Molecular Weight: 45-50 kDa GenBank Accession Number: BC020975 Gene Symbol: TSKU Gene ID (NCBI): 25987 RRID: AB_2877851 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8WUA8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.