Proteintech, 12385-1-AP, UBAP1 Polyclonal antibody

Size: 20ul / 150ul The UBAP1 (12385-1-AP) by Proteintech is a Polyclonal antibody targeting UBAP1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 12385-1-AP targets UBAP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human heart tissue, A431 cells, human lung tissue, human spleen tissue, human testis tissue, mouse brain tissue, mouse lung tissue, mouse testis tissue, mouse thymus tissue, Y79 cells Positive IP detected in: mouse brain tissue Positive IHC detected in: human lung cancer tissue, human heart tissue, human lung tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2400 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information UBAP1, also named as NAG20, is a 55 kDa protein which contains 2 putative tandem UBA domains at the C terminus, a coiled-coil domain, 2 possible N-glycosylation sites, 5 PKC phosphorylation sites, 7 casein kinase II phosphorylation sites, and 3 N-myristoylation sites. UBAP1 might be a potential effective diagnosis candidate for NPC and decreased expression of UBAP1 protein is a possible point of dysfunction along the pathogenesis pathway for NPC that may contribute to malignant transformation. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3055 Product name: Recombinant human UBAP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 203-502 aa of BC020950 Sequence: GGSGSVLQDEEVLASLERATLDFKPLHKPNGFITLPQLGNCEKMSLSSKVSLPPIPAVSNIKSLSFPKLDSDDSNQKTAKLASTFHSTSCLRNGTFQNSLKPSTQSSASELNGHHTLGLSALNLDSGTEMPALTSSQMPSLSVLSVCTEESSPPNTGPTVTPPNFSVSQVPNMPSCPQAYSELQMLSPSERQCVETVVNMGYSYECVLRAMKKKGENIEQILDYLFAHGQLCEKGFDPLLVEEALEMHQCSEEKMMEFLQLMSKFKEMGFELKDIKEVLLLHNNDQDNALEDLMARAGAS Predict reactive species Full Name: ubiquitin associated protein 1 Calculated Molecular Weight: 502 aa, 55 kDa Observed Molecular Weight: 55 kDa GenBank Accession Number: BC020950 Gene Symbol: UBAP1 Gene ID (NCBI): 51271 RRID: AB_2211886 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NZ09 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.