Proteintech, 12479-1-AP, TPP1 Polyclonal antibody

Size: 20ul / 150ul The TPP1 (12479-1-AP) by Proteintech is a Polyclonal antibody targeting TPP1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 12479-1-AP targets TPP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human colon tissue Positive IHC detected in: human colon cancer tissue, mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information TRIPEPTIDYL PEPTIDASE I, TPP1, is a lysosomal exopeptidase that sequentially removes tripeptides from the N termini of proteins. The proenzyme has an apparent molecular mass of 66 kD, and the mature enzyme has an apparent molecular mass of 46 to 48 kD. Defects in TPP1 are the cause of neuronal ceroid lipofuscinosis type 2 (CLN2). This protein distributes widely in all tissues. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3173 Product name: Recombinant human TPP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 215-563 aa of BC014863 Sequence: DVGSGTSNNSQACAQFLEQYFHDSDLAQFMRLFGGNFAHQASVARVVGQQGRGRAGIEASLDVQYLMSAGANISTWVYSSPGRHEGQEPFLQWLMLLSNESALPHVHTVSYGDDEDSLSSAYIQRVNTELMKAAARGLTLLFASGDSGAGCWSVSGRHEFRPTFPASSPYVTTVGGTSFQEPFLITNEIVDYISGGGFSNVFPRPSYQEEAVTKFLSSSPHLPPSSYFNASGRAYPDVAALSDGYWVVSNRVPIPWVSGTSASTPVFGGILSLINEHRILSGRPPLGFLNPRLYQQHGAGLFDVTRGCHESCLDEEVEGQGFCSGPGWDPVTGWGTPNFPALLKTLLNP Predict reactive species Full Name: tripeptidyl peptidase I Calculated Molecular Weight: 563 aa, 61 kDa Observed Molecular Weight: 66 kDa GenBank Accession Number: BC014863 Gene Symbol: TPP1 Gene ID (NCBI): 1200 RRID: AB_2205902 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O14773 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.