Proteintech, 12554-2-AP, PPP2CB Polyclonal antibody

Size: 20ul / 150ul The PPP2CB (12554-2-AP) by Proteintech is a Polyclonal antibody targeting PPP2CB in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 12554-2-AP targets PPP2CB in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse lung tissue, U2OS cells Positive IP detected in: NIH/3T3 cells Positive IHC detected in: rat kidney tissue, human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:250-1:1000 Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3233 Product name: Recombinant human PPP2CB protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-309 aa of BC012022 Sequence: MDDKAFTKELDQWVEQLNECKQLNENQVRTLCEKAKEILTKESNVQEVRCPVTVCGDVHGQFHDLMELFRIGGKSPDTNYLFMGDYVDRGYYSVETVTLLVALKVRYPERITILRGNHESRQITQVYGFYDECLRKYGNANVWKYFTDLFDYLPLTALVDGQIFCLHGGLSPSIDTLDHIRALDRLQEVPHEGPMCDLLWSDPDDRGGWGISPRGAGYTFGQDISETFNHANGLTLVSRAHQLVMEGYNWCHDRNVVTIFSAPNYCYRCGNQAAIMELDDTLKYSFLQFDPAPRRGEPHVTRRTPDYFL Predict reactive species Full Name: protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform Calculated Molecular Weight: 309 aa, 36 kDa Observed Molecular Weight: 36 kDa GenBank Accession Number: BC012022 Gene Symbol: PPP2CB Gene ID (NCBI): 5516 RRID: AB_2169651 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P62714 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.