Proteintech, 12574-1-AP, TSP50 Polyclonal antibody

Size: 20ul / 150ul The TSP50 (12574-1-AP) by Proteintech is a Polyclonal antibody targeting TSP50 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 12574-1-AP targets TSP50 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: MCF7 cells, HEK-293 cells, human testis tissue, MCF-7 cells, mouse testis tissue Positive IHC detected in: human ovary tumor tissue, human breast cancer tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2400 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information TSP50 (Testis-specific protease-like protein 50) is also named as PRSS50, CT20 and belongs to the peptidase S1 family. It can enhance the expression of NFKB target genes involved in cell proliferation, and promotes cell proliferation, at least partially, through activation of the NFKB signalling pathway(PMID:21385156). There are two protein bands of 37 kDa and 43 kDa to be detected in human testes that the the difference is most likely caused by posttranslational proteolytic cleavage(PMID:11782390). The full length protein has a signal peptide with 39 amino acids and two glycosylation sites. Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3247 Product name: Recombinant human TSP50 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 32-385 aa of BC033016 Sequence: LRSAGCWGAGEAPGALSTADPADQSVQCVPKATCPSSRPRLLWQTPTTQTLPSTTMETQFPVSEGKVDPYRSCGFSYEQDPTLRDPEAVARRWPWMVSVRANGTHICAGTIIASQWVLTVAHCLIWRDVIYSVRVGSPWIDQMTQTASDVPVLQVIMHSRYRAQRFWSWVGQANDIGLLKLKQELKYSNYVRPICLPGTDYVLKDHSRCTVTGWGLSKADGMWPQFRTIQEKEVIILNNKECDNFYHNFTKIPTLVQIIKSQMMCAEDTHREKFCYELTGEPLVCSMEGTWYLVGLVSWGAGCQKSEAPPIYLQVSSYQHWIWDCLNGQALALPAPSRTLLLALPLPLSLLAAL Predict reactive species Full Name: testes-specific protease 50 Calculated Molecular Weight: 385 aa, 43 kDa Observed Molecular Weight: 50-55 kDa GenBank Accession Number: BC033016 Gene Symbol: TSP50 Gene ID (NCBI): 29122 RRID: AB_10646475 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UI38 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.