Product Description
Size: 20ul / 150ul
The golgin 97 (12640-1-AP) by Proteintech is a Polyclonal antibody targeting golgin 97 in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples
12640-1-AP targets golgin 97 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.
Tested Applications
Positive WB detected in: HEK-293 cells, SMMC-7721 cells, HeLa cells, HepG2 cells
Positive IF/ICC detected in: HeLa cells
Positive FC (Intra) detected in: HeLa cells
Recommended dilution
Western Blot (WB): WB : 1:2000-1:16000
Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Background Information
Golgin subfamily A member 1 is a protein that in humans is encoded by the GOLGA1 gene. GOLGA1 has been shown to interact with ARL1. GOLGA1 is associated with Sjogren's syndrome. The MW of this protein is 97 kDa, and Catalog#12640-1-AP specially recognises the 97 kDa protein. IF result shows strong Gogli staining.
Specification
Tested Reactivity: human
Cited Reactivity: human, mouse, monkey
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Immunogen: CatNo: Ag3335 Product name: Recombinant human GOLGA1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 418-767 aa of BC032853 Sequence: QIVALERMRAADQTTAEQGMRQLEQENAALKECRNEYERSLQNHQFELKKLKEEWSQREIVSVAMAQALEEVRKQREEFQQQAANLTAIIDEKEQNLREKTEVLLQKEQEILQLERGHNSALLQIHQLQAELEALRTLKAEEAAVVAEQEDLLRLRGPLQAEALSVNESHVTSRAMQDPVFQLPTAGRTPNGEVGAMDLTQLQKEKQDLEQQLLEKNKTIKQMQQRMLELRKTLQKELKIRPDNELFEVREKPGPEMANMAPSVTNNTDLTDAREINFEYLKHVVLKFMSCRESEAFHLIKAVSVLLNFSQEEENMLKETLEYKMSWFGSKPAPKGSIRPSISNPRIPWS Predict reactive species
Full Name: golgi autoantigen, golgin subfamily a, 1
Calculated Molecular Weight: 767 aa, 88 kDa
Observed Molecular Weight: 97 kDa
GenBank Accession Number: BC032853
Gene Symbol: golgin 97
Gene ID (NCBI): 2800
RRID: AB_2115315
Conjugate: Unconjugated
Form: Liquid
Purification Method: Antigen affinity purification
UNIPROT ID: Q92805
Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.
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Collaboration
Tony Tang
Email: Tony.Tang@iright.com
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