Proteintech, 12657-1-AP, Kir7.1 Polyclonal antibody

Size: 20ul / 150ul The Kir7 12657-1-AP targets Kir7.1 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human brain tissue, human kidney tissue Positive IHC detected in: human small intestine tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information Kir7.1, encoded by KCNJ13 gene, is a member of the inwardly rectifying potassium channel family of proteins. Kir7.1 is a low-conductance channel. At mRNA level, Kir7.1 is predominantly expressed in small intestine and also expressed in stomach, kidney, and brain. Mutations in KCNJ13 gene are associated with snowflake vitreoretinal degeneration. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3327 Product name: Recombinant human KCNJ13 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 162-360 aa of BC037290 Sequence: RPKNRAFSIRFTDIAVVAHMDGKPNLIFQVANTRPSPLTSVRVSAVLYQERENGKLYQTSVDFHLDGISSDECPFFIFPLTYYHSITPSSPLATLLQHENPSHFELVVFLSAMQEGTGEICQRRTSYLQSEIMLHHCFASLLTRGSKCEYQIKMENFDKTVPEFPTPLVSKSPNRTDLDIHINGQSIDNFQISETGLTE Predict reactive species Full Name: potassium inwardly-rectifying channel, subfamily J, member 13 Calculated Molecular Weight: 360 aa, 43 kDa Observed Molecular Weight: 43 kDa GenBank Accession Number: BC037290 Gene Symbol: Kir7.1 Gene ID (NCBI): 3769 RRID: AB_2130488 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O60928 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.