Proteintech, 12669-1-AP, CPEB3 Polyclonal antibody

Size: 20ul / 150ul The CPEB3 (12669-1-AP) by Proteintech is a Polyclonal antibody targeting CPEB3 in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse, rat samples 12669-1-AP targets CPEB3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, rat brain tissue Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse brain tissue Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information CPEB3, the cytoplasmic polyadenylation element-binding protein 3, contains a Q/N-rich domain at its N-termina which is required for the aggregation of CPEB3. The expression of CPEB3 is downregulated in cervical cancer and hepatocellular carcinoma compared with normal tissue. It has been reported that the aggregation and activity of CPEB3 is controlled by SUMOylation. CPEB3 is SUMOylated in hippocampal neurons in the basal state while converted into an active form which is associated with a decrease of SUMOylation and an increase of aggregation. CPEB3 is expressed in cytoplasma in unstimulated neurons but translocates to the nucleus following neuronal stimulation. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3351 Product name: Recombinant human CPEB3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 395-698 aa of BC036444 Sequence: FHHPGTDNIMALNNAFLDDSHGDQALSSGLSSPTRCQNGERVERYSRKVFVGGLPPDIDEDEITASFRRFGPLVVDWPHKAESKSYFPPKGYAFLLFQEESSVQALIDACLEEDGKLYLCVSSPIKDKPVQIRPWNLSDSDFVMDGSQPLDPRKTIFVGGVPRPLRAVELAMIMDRLYGGVCYAGIDTDPELKYPKGAGRVAFSNQQSYIAAISARFVQLQHNDIDKRVEVKPYVLDDQMCDECQGTRCGGKFAPFFCANVTCLQYYCEYCWASIHSRAGREFHKPLVKEGGDRPRHVPFRWS Predict reactive species Full Name: cytoplasmic polyadenylation element binding protein 3 Calculated Molecular Weight: 697 aa, 76 kDa Observed Molecular Weight: 76-80 kDa GenBank Accession Number: BC036444 Gene Symbol: CPEB3 Gene ID (NCBI): 22849 RRID: AB_2877871 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8NE35 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.