Proteintech, 12788-1-AP, Calsyntenin-1 Polyclonal antibody

Size: 20ul / 150ul The Calsyntenin-1 (12788-1-AP) by Proteintech is a Polyclonal antibody targeting Calsyntenin-1 in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples 12788-1-AP targets Calsyntenin-1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human brain tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2400 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Calsyntenins (calsyntenin-1, -2, -3) compose a family of neuronally expressed proteins that belong to the cadherin superfamily of cell adhesion molecules (PMID: 24966372; 12498782). Calsyntenin-1 (CLSTN1) is a proteolytically processed postsynaptic membrane protein with a cytoplasmic calcium-binding domain. By binding Ca2+, calsyntenin-1 may modulate Ca2+-mediated postsynaptic signals (PMID: 11161476). Calsyntenin-1 is a transmembrane cargo-docking protein for Kinesin-1-mediated vesicular transport (PMID: 16760430). It can mediate axonal transport of the amyloid precursor protein and regulate Aβ production (PMID: 22434822). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3504 Product name: Recombinant human CLSTN1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 29-350 aa of BC033902 Sequence: ARVNKHKPWLEPTYHGIVTENDNTVLLDPPLIALDKDAPLRFAGEICGFKIHGQNVPFDAVVVDKSTGEGVIRSKEKLDCELQKDYSFTIQAYDCGKGPDGTNVKKSHKATVHIQVNDVNEYAPVFKEKSYKATVIEGKQYDSILRVEAVDADCSPQFSQICSYEIITPDVPFTVDKDGYIKNTEKLNYGKEHQYKLTVTAYDCGKKRATEDVLVKISIKPTCTPGWQGWNNRIEYEPGTGALAVFPNIHLETCDEPVASVQATVELETSHIGKGCDRDTYSEKSLHRLCGAAAGTAELLPSPSGSLNWTMGLPTDNGHDSD Predict reactive species Full Name: calsyntenin 1 Calculated Molecular Weight: 109 kDa Observed Molecular Weight: 109-130 kDa GenBank Accession Number: BC033902 Gene Symbol: Calsyntenin-1 Gene ID (NCBI): 22883 RRID: AB_2229723 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O94985 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.