Proteintech, 12815-1-AP, LRAT Polyclonal antibody

Size: 20ul / 150ul The LRAT (12815-1-AP) by Proteintech is a Polyclonal antibody targeting LRAT in WB, ELISA applications with reactivity to human, mouse, rat samples 12815-1-AP targets LRAT in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse testis tissue, Y79 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Background Information LRAT belongs to the H-rev107 family. LRAT transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters. LRAT plays a critical role in vision. It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, LRAT monomers (26kDa) interact in membranes and form functional homodimers (50-54 kDa) through protein-protein interactions and disulfide bond formation.Defects in LRAT are a cause of severe early-onset retinal dystrophy (RD). This antibody was generated against the internal residues of human LRAT. It is predicted to detect endogenous LRAT. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3556 Product name: Recombinant human LRAT protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 32-201 aa of BC031053 Sequence: EDKGRNSFYETSSFHRGDVLEVPRTHLTHYGIYLGDNRVAHMMPDILLALTDDMGRTQKVVSNKRLILGVIVKVASIRVDTVEDFAYGANILVNHLDESLQKKALLNEEVARRAEKLLGFTPYSLLWNNCEHFVTYCRYGTPISPQSDKFCETVKIIIRDQRSVLASAVL Predict reactive species Full Name: lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase) Calculated Molecular Weight: 230 aa, 26 kDa Observed Molecular Weight: 26 kDa, 50-54 kDa GenBank Accession Number: BC031053 Gene Symbol: LRAT Gene ID (NCBI): 9227 RRID: AB_2297180 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O95237 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.