Proteintech, 12820-1-AP, SLC20A2 Polyclonal antibody

Size: 20ul / 150ul The SLC20A2 (12820-1-AP) by Proteintech is a Polyclonal antibody targeting SLC20A2 in WB, IHC, IF-P, IP, ELISA applications with reactivity to human, mouse samples 12820-1-AP targets SLC20A2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse brain tissue, COLO 320 cells, mouse kidney tissue Positive IP detected in: COLO 320 cells Positive IHC detected in: human bowen disease, mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse brain tissue Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information SLC20A2 (also known as PiT2) is a sodium (Na)-phosphate (Pi) co-transporter that belongs to the SLC20 family. SLC20A2 is highly expressed in the central nervous system and controls hippocampal-dependent learning and memory (PMID: 38195526). SLC20A2 is the major causative gene for primary familial brain calcification (PFBC), a rare neurological disease characterized by widespread cognitive impairment. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3469 Product name: Recombinant human SLC20A2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 231-512 aa of BC028600 Sequence: WLFVCPWMRRKITGKLQKEGALSRVSDESLSKVQEAESPVFKELPGAKANDDSTIPLTGAAGETLGTSEGTSAGSHPRAAYGRALSMTHGSVKSPISNGTFGFDGHTRSDGHVYHTVHKDSGLYKDLLHKIHIDRGPEEKPAQESNYRLLRRNNSYTCYTAAICGLPVHATFRAADSSAPEDSEKLVGDTVSYSKKRLRYDSYSSYCNAVAEAEIEAEEGGVEMKLASELADPDQPREDPAEEEKEEKDAPEVHLLFHFLQVLTACFGSFAHGGNDVSNAIG Predict reactive species Full Name: solute carrier family 20 (phosphate transporter), member 2 Calculated Molecular Weight: 652 aa, 70 kDa Observed Molecular Weight: 68-70 kDa GenBank Accession Number: BC028600 Gene Symbol: SLC20A2 Gene ID (NCBI): 6575 RRID: AB_2191004 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q08357 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.