Proteintech, 12867-1-AP, PHC2 Polyclonal antibody

Size: 20ul / 150ul The PHC2 (12867-1-AP) by Proteintech is a Polyclonal antibody targeting PHC2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 12867-1-AP targets PHC2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A375 cells, A549 cells Positive IP detected in: HL-60 cells Positive IHC detected in: human pancreas cancer tissue, mouse kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A549 cells, A375 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Specification Tested Reactivity: human, mouse, rat Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3507 Product name: Recombinant human PHC2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-323 aa of BC028396 Sequence: MTSGNGNSASSIAGTAPQNGENKPPQAIVKPQILTHVIEGFVIQEGAEPFPVGRSSLLVGNLKKKYAQGFLPEKLPQQDHTTTTDSEMEEPYLQESKEEGAPLKLKCELCGRVDFAYKFKRSKRFCSMACAKRYNVGCTKRVGLFHSDRSKLQKAGAATHNRRRASKASLPPLTKDTKKQPTGTVPLSVTAALQLTHSQEDSSRCSDNSSYEEPLSPISASSSTSRRRQGQRDLELPDMHMRDLVGMGHHFLPSEPTKWNVEDVYEFIRSLPGCQEIEEEFRAQEIDGQALLLLKEDHLMSAMNIKLGPALKIYARISMLKDS Predict reactive species Full Name: polyhomeotic homolog 2 (Drosophila) Calculated Molecular Weight: 858 aa, 91 kDa Observed Molecular Weight: 40 kDa GenBank Accession Number: BC028396 Gene Symbol: PHC2 Gene ID (NCBI): 1912 RRID: AB_2877890 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8IXK0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.