Proteintech, 12882-1-AP, SH2D3C Polyclonal antibody

Size: 20ul / 150ul The SH2D3C (12882-1-AP) by Proteintech is a Polyclonal antibody targeting SH2D3C in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples 12882-1-AP targets SH2D3C in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, human brain tissue, human placenta tissue Positive IP detected in: mouse brain tissue, Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2400 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information SH2 domain-containing protein 3C (SH2D3C, or NSP3) gene encodes an adaptor protein and member of a cytoplasmic protein family involved in cell migration. The encoded protein was identified as an Eph receptor-binding protein which may be a positive regulator of TCR signaling (PMID: 10542222). SH2D3C is an adaptor protein that has been implicated in T cell activation and shown to associate with different components of the integrin signaling pathway ex vivo, moreover, its primary in vivo role of Sh2d3c in regulating B cell development and function has also been demonstrated (PMID: 20505138). SH2D3C has 6 isoforms with MW 94, 86, 77 and 55 kDa (refer to UniProt). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3887 Product name: Recombinant human SH2D3C protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 354-703 aa of BC027962 Sequence: TETSSQQARSYGERLKELSENGAPEGDWGKTFTVPIVEVTSSFNPATFQSLLIPRDNRPLEVGLLRKVKELLAEVDARTLARHVTKVDCLVARILGVTKEMQTLMGVRWGMELLTLPHGRQLRLDLLERFHTMSIMLAVDILGCTGSAEERAALLHKTIQLAAELRGTMGNMFSFAAVMGALDMAQISRLEQTWVTLRQRHTEGAILYEKKLKPFLKSLNEGKEGPPLSNTTFPHVLPLITLLECDSAPPEGPEPWGSTEHGVEVVLAHLEAARTVAHHGGLYHTNAEVKLQGFQARPELLEVFSTEFQMRLLWGSQGASSSQARRYEKFDKVLTALSHKLEPAVRSSEL Predict reactive species Full Name: SH2 domain containing 3C Calculated Molecular Weight: 94 kDa, 86 kDa, 77 kDa Observed Molecular Weight: 77 kDa GenBank Accession Number: BC027962 Gene Symbol: SH2D3C Gene ID (NCBI): 10044 RRID: AB_2188993 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8N5H7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.