Proteintech, 12936-1-AP, NFE2L1 Polyclonal antibody

Size: 20ul / 150ul The NFE2L1 (12936-1-AP) by Proteintech is a Polyclonal antibody targeting NFE2L1 in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples 12936-1-AP targets NFE2L1 in WB, IHC, IF, IP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse skeletal muscle tissue, Fetal mouse bone tissue, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells Positive IP detected in: mouse skeletal muscle tissue Positive IHC detected in: human breast cancer tissue, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information NFE2L1, also named as HBZ17, NRF1, TCF11 and LCR-F1, belongs to the bZIP family and CNC subfamily. It activates erythroid-specific, globin gene expression. NFE2L1 is involved in mediating ascorbic acid-induced osterix expression and osteoblast differentiation via binding to the antioxidant response element of the osterix promoter. NFE2L1, produced by osteoblasts, is involved in regulating osterix expression, osteoblast differentiation, and bone formation. (PMID:19887580). This antibody is a rabbit polyclonal antibody raised against a region of human NFE2L1. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3916 Product name: Recombinant human NFE2L1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 293-742 aa of BC010623 Sequence: MQAMEVNTSASEILYSAPPGDPLSTNYSLAPNTPINQNVSLHQASLGGCSQDFLLFSPEVESLPVASSSTLLPLAPSNSTSLNSTFGSTNLTGLFFPPQLNGTANDTAGPELPDPLGGLLDEAMLDEISLMDLAIEEGFNPVQASQLEEEFDSDSGLSLDSSHSPSSLSSSEGSSSSSSSSSSSSSSASSSASSSFSEEGAVGYSSDSETLDLEEAEGAVGYQPEYSKFCRMSYQDPAQLSCLPYLEHVGHNHTYNMAPSALDSADLPPPSALKKGSKEKQADFLDKQMSRDEHRARAMKIPFTNDKIINLPVEEFNELLSKYQLSEAQLSLIRDIRRRGKNKMAAQNCRKRKLDTILNLERDVEDLQRDKARLLREKVEFLRSLRQMKQKVQSLYQEVFGRLRDENGRPYSPSQYALQYAGDGSVLLIPRTMADQQARRQERKPKDRRK Predict reactive species Full Name: nuclear factor (erythroid-derived 2)-like 1 Calculated Molecular Weight: 772 aa, 85 kDa Observed Molecular Weight: 65 kDa GenBank Accession Number: BC010623 Gene Symbol: NFE2L1 Gene ID (NCBI): 4779 RRID: AB_2267298 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q14494 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.