Proteintech, 12995-1-AP, NEU4 Polyclonal antibody

Size: 20ul / 150ul The NEU4 (12995-1-AP) by Proteintech is a Polyclonal antibody targeting NEU4 in WB, IHC, IP, ELISA applications with reactivity to human samples 12995-1-AP targets NEU4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: COLO 320 cells, HepG2 cells, L02 cells Positive IP detected in: L02 cells Positive IHC detected in: human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information NEU4, also named as LP5125, belongs to a family of glycohydrolytic enzymes, which remove terminal sialic acid residues from various sialo derivatives, such as glycoproteins, glycolipids, oligosaccharides, and gangliosides. It may function in lysosomal catabolism of sialylated glycoconjugates. NEU4 has a broad substrate specificity being active on glycoproteins, oligosaccharides and sialylated glycolipids. Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3657 Product name: Recombinant human NEU4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 158-496 aa of BC012899 Sequence: AIGGAVQDWATFAVGPGHGVQLPSGRLLVPAYTYRVDRRECFGKICRTSPHSFAFYSDDHGRTWRCGGLVPNLRSGECQLAAVDGGQAGSFLYCNARSPLGSRVQALSTDEGTSFLPAERVASLPETAWGCQGSIVGFPAPAPNRPRDDSWSVGPGSPLQPPLLGPGVHEPPEEAAVDPRGGQVPGGPFSRLQPRGDGPRQPGPRPGVSGDVGSWTLALPMPFAAPPQSPTWLLYSHPVGRRARLHMGIRLSQSPLDPRSWTEPWVIYEGPSGYSDLASIGPAPEGGLVFACLYESGARTSYDEISFCTFSLREVLENVPASPKPPNLGDKPRGCCWPS Predict reactive species Full Name: sialidase 4 Calculated Molecular Weight: 496 aa, 53 kDa Observed Molecular Weight: 52-55 kDa GenBank Accession Number: BC012899 Gene Symbol: NEU4 Gene ID (NCBI): 129807 RRID: AB_2149206 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8WWR8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.