Proteintech, 13103-2-AP, TSEN2 Polyclonal antibody

Size: 20ul / 150ul The TSEN2 (13103-2-AP) by Proteintech is a Polyclonal antibody targeting TSEN2 in WB, IF/ICC, ELISA applications with reactivity to human, mouse samples 13103-2-AP targets TSEN2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse colon tissue, human brain tissue, mouse brain tissue Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information TSEN2, also named as SEN2, belongs to the tRNA-intron endonuclease family. TSEN2 is a metal ion independent nuclease that cleave the 5' splice site (PMID: 32476018). TSEN2 has 5 isoforms with the molecular mass of 45-53 kDa. TSEN2 is involved in the formation of a human endonuclease complex whose function is the removal of introns from pre-tRNA (PMID: 16595010). Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3742 Product name: Recombinant human TSEN2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 159-465 aa of BC019582 Sequence: SNMEGTAGGERPSVVNGDSGKSGGVGDPREPLGCLQEGSGCHPTTESFEKSVREDASPLPHVCCCKQDALILQRGLHHEDGSQHIGLLHPGDRGPDHEYVLVEEAECAMSEREAAPNEELVQRNRLICRRNPYRIFEYLQLSLEEAFFLVYALGCLSIYYEKEPLTIVKLWKAFTVVQPTFRTTYMAYHYFRSKGWVPKVGLKYGTDLLLYRKGPPFYHASYSVIIELVDDHFEGSLRRPLSWKSLAALSRVSVNVSKELMLCYLIKPSTMTDKEMESPECMKRIKVQEVILSRWVSSRERSDQDDL Predict reactive species Full Name: tRNA splicing endonuclease 2 homolog (S. cerevisiae) Calculated Molecular Weight: 465 aa, 51 kDa Observed Molecular Weight: 51 kDa GenBank Accession Number: BC019582 Gene Symbol: TSEN2 Gene ID (NCBI): 80746 RRID: AB_2272213 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8NCE0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.