Proteintech, 13273-1-AP, DAO Polyclonal antibody

Size: 20ul / 150ul The DAO (13273-1-AP) by Proteintech is a Polyclonal antibody targeting DAO in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 13273-1-AP targets DAO in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse kidney tissue, rat cerebellum tissue, A549 cells Positive IHC detected in: human colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:100-1:400 Background Information D-amino acid oxidase (DAO),with MW of 39 kDa, is a flavoprotein which uses flavin adenine dinucleotide as its prosthetic group. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. DAO may play a role in the pathophysiology of schizophrenia and act as a detoxifying agent which removes D-amino acids that accumulate during aging. DAAO exists in solution as a mixture of monomer-dimer(80 kDa) and even higher molecular aggregates in equilibrium(170 kDa), depending on the enzyme concentration and forms(PMID: 11130179/PMID: 9210639). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3973 Product name: Recombinant human DAO protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 24-347 aa of BC029057 Sequence: HSVLQPLDIKVYADRFTPLTTTDVAAGLWQPYLSDPNNPQEADWSQQTFDYLLSHVHSPNAENLGLFLISGYNLFHEAIPDPSWKDTVLGFRKLTPRELDMFPDYGYGWFHTSLILEGKNYLQWLTERLTERGVKFFQRKVESFEEVAREGADVIVNCTGVWAGALQRDPLLQPGRGQIMKVDAPWMKHFILTHDPERGIYNSPYIIPGTQTVTLGGIFQLGNWSELNNIQDHNTIWEGCCRLEPTLKNARIIGERTGFRPVRPQIRLEREQLRTGPSNTEVIHNYGHGGYGLTIHWGCALEAAKLFGRILEEKKLSRMPPSHL Predict reactive species Full Name: D-amino-acid oxidase Calculated Molecular Weight: 347 aa, 39 kDa Observed Molecular Weight: 39 kDa GenBank Accession Number: BC029057 Gene Symbol: DAO Gene ID (NCBI): 1610 RRID: AB_2877932 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P14920 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.